This formulation is compatible with up to 1% of commonly used detergents. Using standard procedure, the assay is used with samples having protein concentrations between 200 and 1,400 µg/ml (20–140 µg total). The assay supplies ready-to-use dye reagent at 1x concentration and two protein standards at seven prediluted concentrations (0.125, 0.25, 0.5, 0.75, 1.0, 1.5, and 2.0 mg/ml). * Concentrations compatible with the microassays are 1/25 of the values listed above. Detergent Compatible Bradford Assay Reagent, 450 mL, store at 4°C, Protein Quantitation Assay Kits, Reagents & Standards, Dosage Bradford compatible avec le détergent Pierce™, Gérer l'utilisation, l'information et le service de l'instrument, Consommables en plastique de culture cellulaire, Voir les liens pour Applications et techniques, Extraction et analyse de l’ADN et de l’ARN, Solutions pour les sociétés de biotechnologie, Recherche pharmaceutique et développement de médicaments, Industries pharmaceutiques et biopharmaceutiques, Spectroscopie, analyse élémentaire et isotopique, Développement du diagnostic préclinique au diagnostic compagnon, Logiciels de gestion et d’analyse de données de laboratoire, Consommables en plastique et matériel de laboratoire, Réactifs de culture cellulaire et de transfection, Colonnes de chromatographie, résines et filtres de centrifugation, Réactifs de laboratoire et produits chimiques, Fournitures, consommables en plastique et en verre pour laboratoire, Amorces/oligos, clonage et synthèse des gènes, Informatique de laboratoire à l’échelle de l’entreprise, OEM & Commercial SupplyLicences et offres commerciales, Certifications ISO du site de fabrication, Notions fondamentales en culture cellulaire Gibco, Lettres d’information électroniques et journaux, Plate-forme d’outils et d’utilitaires pour oligos, Données chiffrées utiles pour la culture cellulaire, Générateur de panels de cytométrie en flux, Outil Switch-to-Nunc pour les supports de culture, Calculateur de protocoles de transfection, Applications de bureau et applications mobiles. Standard-concentration assay : Sample volume: 100 µl: Linear range: 0.125-1.5 mg/ml: Low-concentration assay : Sample volume: 1 ml: Linear range: 1.25-25 µg/ml: Assay wavelength: 595 nm: Minimum incubation time: 5 min : Adapted from method of: Bradford: x. … Product Description: Bradford Protein Assay Kit: Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595 nm. Dilution may also be required in the presence of flavonoids and some basic buffers, as they can also interfere with Bradford protein assays. email to, 5000201Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays. You can use the batch number in place of the lot/control number. The Bio-Rad protein assay is a simple colorimetric assay for measuring total protein concentration and is based on the Bradford dye-binding method (Bradford 1976). The following replacement is recommended: 5000204Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml), 5000209Pkg of 2, set of 7 protein concentrations standards, 0.125–2.0 mg/ml, for use with Quick Start Bradford protein assay kits, 50002065 x 2 ml vials, 2 mg/ml, prediluted protein concentration standard for use with Quick Start Bradford protein assay kits, 5000207Pkg of 2, set of 7 protein concentrations standards, 0.125–2.0 mg/ml, for use with Quick Start Bradford protein assay kits, 50002085 x 2 ml vials, 2 mg/ml, prediluted standard for use with Quick Start Bradford protein assay kits, 50002051 L, ready-to-use dye reagent for use with Quick Start Bradford protein assay kits, This product contains electrical or electronic material. However, for some reason, the actual file itself is unavailable. If a quick Bradford protein assay kit with a ready-to-use Bradford reagent is used, detergents that interfere will need to be at a lower concentration in the sample than in the standard assay due to the high sample-to-dye ratio. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date. This kit is designed to quantitate 10-150 µg/ml of protein. It is easy to adapt the assay from the standard-concentration range to … Bradford Protein Assay … Not for use in diagnostic procedures. See the sample label below for the location of this information. Account price failed to display. There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. please email us your inquiry at: Request a Free Sample* at: order@biobasic.com, For product support/troubleshooting, If you are an educator at the K–12 or college level, contact your local Customer Service office to establish an education account number. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. No mixing or … Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile. This assay works by measuring the color change achieved with the basic amino acids … Not sure? Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. tech@biobasic.com, CE-IVD Certified COVID-19 Kits, Probes & more, Summer Savings - Genes $0.09/bp (CAD $0.12), 20 Konrad Crescent, Markham ON, Canada, L3R 8T4 | 4160 Bailey Avenue, Amherst, NY, USA, 14226 | Copyright © 2017 Bio Basic Inc. All rights reserved, Modified Lowry Protein Assay Kit (Improved accuracy), Better BCA Protein Assay Kit (Reducing agent compatible), Non-Interfering Protein Concentration Determination Kit, You may also be interested in the following product(s).
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